Single Cell Isolation Microfluidics

Dielectrophoretic digital sorting enzymatic digestion facs hydrodynamic traps laser capture microdissection manual picking microfluidics micromanipulation serial dilution and raman tweezers.

Single cell isolation microfluidics.

Methods currently used for single cell isolation include. The unique droplet microfluidic technology speeds up the process at the step of isolating activated single antibody secreting cells in picoliter volume chambers from various tissues including blood bone marrow and b cell plasma which allows secreted antibodies to be detected within hours with a variety of bead based and cell based assay formats. Here we present a microfluidic system that can overcome these limitations making use of a dual color signal normalization approach. We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential.

131 132 in parallel this technology has been developed commercially by gigagen. Many single cell analysis techniques require the isolation of individual cells. Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system. It refers to the process of isolation manipulation and analysis of the cells at single cell resolution in microfluidic chips.

Single cell analysis has become one of the most important applications of microfluidic technology in recent years. Depending on the application single cell analysis chips can include any or all the above mentioned steps.